Research Plans: Testing the model
 
 
Ongoing Projects and Collaborations: 1. Mechanisms of CENP-A assembly a) Role of UNG2 in CENP-A assembly Funded by NIH F32 GM069297 (2003-2006) Recent Collaborators: Geir Slupphaug (NTNU, Norway) Jean Wang (UCSD and Moores Cancer Center) Publications: Zeitlin SG et al. DNA Repair (2005) Zeitlin SG et al. (submitted, 2007) b) CENP-A dynamics and protein stability 2. Role of CENP-A in DNA repair Recent Collaborators: Don Cleveland (LICR and UCSD) Michael Berns (UCSD and UC Irvine) Publications and Presentations: Zeitlin SG et al. PNAS (2009) presented at American Society of Biochemistry and Molecular Biology at FASEB 2008, 2009 presented at Keystone meeting on Chromatin Dynamics and Higher Order Organization 2009 presented at Keystone meeting on Genome Instability and DNA Repair 2009 presented at the ASCB Annual Meeting in San Diego, Sunday December 6, 2009 9:15 AM Minisymposium 4: Chromatin Dynamics and Organization (Ballroom 20 B/C) 3. Consequences of CENP-A mislocalization in human disease Cancer: Disease consequences and predictive utility of CENP-A overexpression in cancer collaborator Susan McGovern, MD, PhD (MD Anderson) Nutrition: CENP-A mislocalization as a result of folate deficiency Stem cells and development: a) Neurodegeneration Mechanisms and consequences of CENP-A mislocalization in patients with Ataxia Telangiectasia Funded by California Institute for Regenerative Medicine (2006-2007) collaborators Richard Gatti, MD (UCLA) and York Marahrens, PhD (University of Minnesota) b) Immunology CENP-A localization and function in B-cell maturation http://crisp.cit.nih.gov/crisp/CRISP_LIB.getdoc?textkey=6695938&p_grant_num=1F32GM069297-01&p_query=&ticket=100504720&p_audit_session_id=481615482&p_keywords=http://www.ntnu.no/dmf/ikm/molbiol/dnarep/proteomikk/englishhttp://cancer.ucsd.edu/wanglabhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6X17-4GDK9WP-1&_coverDate=07%2F12%2F2005&_alid=434935026&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=7235&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=78c27a9f7ac55103d0b8b2e2bd4b11a9http://cmm.ucsd.edu:16080/clevelandhttp://www.robolase.ucsd.edu/home.htmlhttp://www.pnas.org/content/106/37/15762.shorthttp://www.keystonesymposia.org/Meetings/viewPastMeetings.cfm?MeetingID=1000&CFID=3325842&CFTOKEN=84031865http://www.keystonesymposia.org/Meetings/viewPastMeetings.cfm?MeetingID=979&CFID=3325842&CFTOKEN=84031865http://www.ascb.org/meetings/http://www.cirm.ca.gov/http://www.mrrc.npi.ucla.edu/mrrc/faculty.asp?faculty=1445http://www.gcd.umn.edu/html/faculty_pages/marahrens.htmlshapeimage_2_link_0shapeimage_2_link_1shapeimage_2_link_2shapeimage_2_link_3shapeimage_2_link_4shapeimage_2_link_5shapeimage_2_link_6shapeimage_2_link_7shapeimage_2_link_8shapeimage_2_link_9shapeimage_2_link_10shapeimage_2_link_11shapeimage_2_link_12
Aim: Test the role of APE in recruiting CENP-A to centromeres
Aim: Characterize the biochemical function of CENP-A in DNA repair
Aim: Identify the human cytidine deaminase acting at centromeres (12 candidate enzymes in the human genome)
Aim: Apply DNA damage to generate neocentromeres
Aim: Characterize the feedback mechanism of CENP-A proteolytic restriction to centromeres